Oxygen Transport and Unloading
1. From Lungs to Blood:
- Inhalation: During inhalation, air containing oxygen enters the alveoli, where the partial pressure of oxygen (PO₂) is high (~100 mmHg).
- Diffusion: Oxygen diffuses from the alveoli into the blood in the pulmonary capillaries due to the concentration gradient (high PO₂ in alveoli, low PO₂ in blood).
- Binding to Hemoglobin:
O2 + Hb → HbO2
(Oxygen + Hemoglobin → Oxyhemoglobin)
2. Transport in Blood:
- Oxyhemoglobin: Most oxygen is transported bound to haemoglobin (Hb), forming oxyhemoglobin (HbO₂).
- Dissolved Oxygen: A small percentage (~2%) of oxygen is dissolved in the blood plasma.
3. From Blood to Tissues (Unloading of Oxygen):
- Oxygen Release Mechanism:
The release of oxygen from haemoglobin is influenced by several factors described by the Bohr effect: - a. Partial Pressure of Oxygen (PO₂):
- In tissues, the PO₂ is lower (~40 mmHg) than in the blood (~100 mmHg). This gradient drives the diffusion of oxygen from blood into tissues.
- b. Partial Pressure of Carbon Dioxide (PCO₂):
- In tissues, PCO₂ is higher due to cellular respiration. CO₂ diffuses into the blood, increasing local PCO₂, which promotes oxygen unloading.
CO2 + H2O → H2CO3 →. H+ + HCO3−
Carbon Dioxide + Water → Carbonic Acid → Hydrogen Ion + Bicarbonate Ion - c. pH (Bohr Effect):
- Increased CO₂ in tissues leads to the production of H⁺ ions (lower pH), reducing haemoglobin’s affinity for oxygen and promoting its release.
HbO2 + H+ → HHb + O2
(Oxyhemoglobin + Hydrogen Ion → Reduced Hemoglobin + Oxygen) - d. Temperature:
- Active tissues produce heat, increasing local temperature, which decreases haemoglobin’s affinity for oxygen, enhancing oxygen release.
- e. 2,3-Bisphosphoglycerate (2,3-BPG):
- 2,3-BPG, a byproduct of glycolysis in red blood cells, binds to deoxygenated haemoglobin, reducing its affinity for oxygen and facilitating oxygen release.
4. Cellular Respiration:
- Oxygen diffuses into cells and is used in the mitochondria for aerobic respiration to produce ATP:
C6H12O6 + 6O2 → 6CO2 + 6H2O + Energy (ATP)
Glucose + Oxygen → Carbon Dioxide + Water + Energy
Carbon Dioxide Transport
1. From Tissues to Blood:
- Production: CO₂ is produced as a byproduct of cellular metabolism, leading to higher PCO₂ in the tissues (~45 mmHg) compared to the blood (~40 mmHg).
- Diffusion into Blood: CO₂ diffuses from the tissues into the blood in the systemic capillaries due to the concentration gradient.
2. Transport in Blood:
- Dissolved in Plasma: About 7-10% of CO₂ is transported dissolved directly in the plasma.
- Carbaminohaemoglobin:
CO2 + Hb → HbCO2
(Carbon Dioxide + Hemoglobin → Carbaminohemoglobin) - Bicarbonate Ions:
CO2 + H2O → H2CO3 → H+ + HCO3−
(Carbon Dioxide + Water → Carbonic Acid → Hydrogen Ion + Bicarbonate Ion) - CO₂ reacts with water inside red blood cells, catalysed by carbonic anhydrase, forming carbonic acid (H₂CO₃), which quickly dissociates into bicarbonate (HCO₃⁻) and hydrogen (H⁺) ions.
- Bicarbonate ions diffuse out of red blood cells into the plasma in exchange for chloride ions (Cl⁻) (chloride shift).
3. From Blood to Lungs:
- Return to Lungs: Deoxygenated blood carrying CO₂ returns to the right side of the heart and is then pumped to the lungs via the pulmonary arteries.
- Reverse Reactions in Lungs:
H+ +. HCO3− →. H2CO3 →. CO2 +. H2O
(Hydrogen Ion + Bicarbonate Ion → Carbonic Acid → Carbon Dioxide + Water) - Bicarbonate ions re-enter red blood cells and recombine with hydrogen ions to form carbonic acid, which is then converted back into CO₂ and water by carbonic anhydrase.
- CO₂ is released from carbamino-haemoglobin.
4. Exhalation:
- Diffusion into Alveoli: CO₂ diffuses from the blood (high PCO₂) into the alveoli (low PCO₂) due to the concentration gradient.
- Exhalation: CO₂ is expelled from the lungs during exhalation, completing the respiratory cycle.
Summary
- Oxygen Transport: Inhaled oxygen diffuses into the blood, binds to haemoglobin, and is transported to tissues where it diffuses into cells for cellular respiration.
- Oxygen Unloading: Facilitated by lower PO₂, higher PCO₂, lower pH (Bohr effect), higher temperature, and the presence of 2,3-BPG.
- CO₂ Transport: CO₂ produced by cellular respiration diffuses into the blood, is transported dissolved in plasma, bound to haemoglobin, or as bicarbonate ions, and is ultimately exhaled from the lungs.